验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, mouse kidney tissue, rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34064-1-AP targets VPS9D1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag41163 Product name: Recombinant human C16orf7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 300-393 aa of BC148701 Sequence: YPAVSRAAAPAPGCCPPTPNPGSRRLRPSQSLHCMLSPPEPSAAPRPQDSPPTPPLQPGPVGSPSPLGDTASGLPDKDSSFEDLEQFLGTSERQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 16 open reading frame 7 |
| 别名 | ATPBL, C16orf7, Protein ATP-BL, VPS9 domain-containing protein 1 |
| 计算分子量 | 73 kDa |
| 观测分子量 | 73 kDa |
| GenBank蛋白编号 | BC148701 |
| 基因名称 | C16orf7 |
| Gene ID (NCBI) | 9605 |
| RRID | AB_3743148 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Y2B5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS9 domain-containing protein 1 (VPS9D1) promotes tubular endosome formation by specifically activating Rab22A (PMID: 36762583). The long non-coding RNA VPS9D1-AS1 can be activated by CEBPB transcription factor and target VEGFA to activate its downstream pathway to promote colorectal cancer angiogenesis, which may suggest that VPS9D1-AS1 is critical for regulating colorectal cancer angiogenesis (PMID: 40371141).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS9D1 antibody 34064-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
