验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human testis tissue, mouse brain tissue, mouse liver tissue, PC-3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15079-1-AP targets VPS8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag7151 Product name: Recombinant human VPS8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 56-147 aa of BC001001 Sequence: MSPSYHQSKGDPTAKKGTSEPVLDPQQIQAFDQLCRLYRGSSRLALLTELSQNRSSESYRPFSGSQSAPAFNSIFQNENFQLQLIPPPVTED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 8 homolog (S. cerevisiae) |
| 别名 | KIAA0804, Vacuolar protein sorting-associated protein 8 homolog |
| 计算分子量 | 162 kDa |
| 观测分子量 | 151 kDa |
| GenBank蛋白编号 | BC001001 |
| 基因名称 | VPS8 |
| Gene ID (NCBI) | 23355 |
| RRID | AB_2215543 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N3P4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS8, also named as KIAA0804, has some isoforms with MW 135-160 kDa and 70-90 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for VPS8 antibody 15079-1-AP | Download protocol |
| IP protocol for VPS8 antibody 15079-1-AP | Download protocol |
| WB protocol for VPS8 antibody 15079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.
| ||
Traffic Mammalian CORVET is required for fusion and conversion of distinct early endosome sub-populations. | ||
Sci Rep HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosis
|
