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验证数据展示

  • WB analysis of SH-SY5Y using 67404-1-Ig (same clone as 67404-1-PBS)

    WB analysis of SH-SY5Y using 67404-1-Ig (same clone as 67404-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

    IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

    IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51221-2

    Cytometric bead array standard curve of MP51221-2

    Cytometric bead array standard curve of MP51221-2, VPS54 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67404-2-PBS. Detection antibody: 67404-1-PBS. Standard:Ag4123. Range: 0.195-25 ng/mL.

  • Cytometric bead array standard curve of MP51221-3

    Cytometric bead array standard curve of MP51221-3

    Cytometric bead array standard curve of MP51221-3, VPS54 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67404-3-PBS. Detection antibody: 67404-1-PBS. Standard:Ag4123. Range: 0.195-12.5 ng/mL.

VPS54 Monoclonal antibody, PBS Only (Detector)

VPS54 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 67404-1-PBS

产品说明书

CloneNo. 3D12D1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Vacuolar protein sorting-associated protein 54, Tumor antigen SLP-8p, Tumor antigen HOM-HCC-8, Hepatocellular carcinoma protein 8, HCC8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67404-1-PBS targets VPS54 as part of a matched antibody pair:

MP51221-2: 67404-2-PBS capture and 67404-1-PBS detection (validated in Cytometric bead array)

MP51221-3: 67404-3-PBS capture and 67404-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 VPS54 fusion protein Ag4123 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 54 homolog (S. cerevisiae)
别名 Vacuolar protein sorting-associated protein 54, Tumor antigen SLP-8p, Tumor antigen HOM-HCC-8, Hepatocellular carcinoma protein 8, HCC8
计算分子量 977 aa, 111 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC030275
基因名称 VPS54
Gene ID (NCBI) 51542
RRIDAB_2882645
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9P1Q0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.