验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, human kidney tissue, mouse brain tissue, rat pancreas tissue, rat brain tissue, rat liver tissue |
| Positive IP detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
12616-1-AP targets VPS45 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3289 Product name: Recombinant human VPS45 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 271-570 aa of BC028382 Sequence: FYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEVLASGLHSRSKESSQVTSRSASRR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 45 homolog (S. cerevisiae) |
| 别名 | h VPS45, H1, H1VPS45, hlVps45, h-VPS45 |
| 计算分子量 | 570 aa, 65 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC028382 |
| 基因名称 | VPS45 |
| Gene ID (NCBI) | 11311 |
| RRID | AB_2215367 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NRW7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferPHVal}} |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for VPS45 antibody 12616-1-AP | Download protocol |
| WB protocol for VPS45 antibody 12616-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Gastroenterol VPS45 promotes the progression of hepatocellular carcinoma by recycling β1 integrin to the cell membrane via the endocytic pathway.
| ||
Sci Rep Predictive effect and clinical diagnosis significance of exosome-related genes for nonalcoholic fatty liver disease-related hepatocellular carcinoma. | ||
J Cell Sci The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes. | ||
Mol Carcinog VPS45 Contributes to the Progression of Hepatocellular Carcinoma by Triggering the Wnt/β-Catenin Signaling Pathway | ||
Nat Commun In vivo CRISPR screening identifies metastasis suppressors in triple-negative breast cancer. |
