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验证数据展示

  • WB analysis using 27128-1-AP

    WB analysis using 27128-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27128-1-AP (VPS37C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 27128-1-AP

    IHC staining of mouse colon using 27128-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27128-1-AP (VPS37C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 27128-1-AP

    IHC staining of mouse lung using 27128-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 27128-1-AP (VPS37C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Raji using 27128-1-AP

    IF Staining of Raji using 27128-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using VPS37C antibody (27128-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

VPS37C Polyclonal antibody

VPS37C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27128-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ESCRT I complex subunit VPS37C, ESCRT-I complex subunit VPS37C, hVps37C, PML39, Vacuolar protein sorting-associated protein 37C

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, SK-BR-3 cells, T-47D cells
Positive IHC detected inmouse colon tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27128-1-AP targets VPS37C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26024

Product name: Recombinant human VPS37C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-117 aa of BC141827

Sequence: METLKDKTLQELEELQNDSEAIDQLALESPEVQDLQLEREMALATNRSLAERNLEFQGPLEISRSNLSDRYQELRKLVERCQEQKAKLEKFSSALQPGTLLDLLQVEGMKIEEESEA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 37 homolog C (S. cerevisiae)
别名 ESCRT I complex subunit VPS37C, ESCRT-I complex subunit VPS37C, hVps37C, PML39, Vacuolar protein sorting-associated protein 37C
观测分子量 39-45 kDa
GenBank蛋白编号BC141827
基因名称 VPS37C
Gene ID (NCBI) 55048
RRIDAB_3085928
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA5D8V6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS37C (Vacuolar protein sorting-associated protein 37C) is also named as hVps37C, PML39 and ESCRT-I complex subunit VPS37C. VPS37C is a core subunit of the Endosomal sorting complex required for transport-I (ESCRT-I) complex (PMID: 37307706). VPS37C interacts with EKLF and prevents K48-linked polyubiquitination of EKLF and proteasome-mediated EKLF degradation, thus enhancing EKLF protein stability and transcriptional activity (PMID: 37307706).

实验方案

Product Specific Protocols
IF protocol for VPS37C antibody 27128-1-APDownload protocol
IHC protocol for VPS37C antibody 27128-1-APDownload protocol
WB protocol for VPS37C antibody 27128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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