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验证数据展示

  • WB analysis using 29387-1-AP (same clone as 29387-1-PBS)

    WB analysis using 29387-1-AP (same clone as 29387-1-PBS)

    HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 29387-1-AP (VPS13D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29387-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 29387-1-AP (same clone as 29387-1-PBS)

    IHC staining of human ovary cancer using 29387-1-AP (same clone as 29387-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29387-1-AP (VPS13D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29387-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 29387-1-AP (same clone as 29387-1-PBS)

    IF Staining of U2OS using 29387-1-AP (same clone as 29387-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using VPS13D antibody (29387-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29387-1-PBS in a different storage buffer formulation.

VPS13D Polyclonal antibody, PBS Only

VPS13D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29387-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Intermembrane lipid transfer protein VPS13D, KIAA0453, Vacuolar protein sorting-associated protein 13D

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29387-1-PBS targets VPS13D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29933

Product name: Recombinant human VPS13D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 4202-4388 aa of NM_015378

Sequence: DFASETAQAVRDTATLSGPRTQAQRVRKPRCCTGPQGLLPRYSESQAEGQEQLFKLTDNIQDEFFIAVENIDSYCVLISSKAVYFLKSGDYVDREAIFLEVKYDDLYHCLVSKDHGKVYVQVTKKAVSTSSGVSIPGPSHQKPMVHVKSEVLAVKLSQEINYAKSLYYEQQLMLRLSENREQLELDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 13 homolog D (S. cerevisiae)
别名 Intermembrane lipid transfer protein VPS13D, KIAA0453, Vacuolar protein sorting-associated protein 13D
计算分子量 492 kDa
观测分子量~500 kDa
GenBank蛋白编号NM_015378
基因名称 VPS13D
Gene ID (NCBI) 55187
RRIDAB_3086126
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5THJ4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS13 (vacuolar protein sorting 13 ) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

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