验证数据展示
经过测试的应用
| Positive WB detected in | Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
11915-1-AP targets VPREB3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag2528 Product name: Recombinant human VPREB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-123 aa of BC020666 Sequence: MACRCLSFLLMGTFLSVSQTVLAQLDALLVFPGQVAQLSCTLSPQHVTIRDYGVSWYQQRAGSAPRYLLYYRSEEDHHRPADIPDRFSAAKDEAHNACVLTISPVQPEDDADYYCSVGYGFSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pre-B lymphocyte 3 |
| 别名 | 8HS20, N27C7 2, pre B lymphocyte 3, Pre B lymphocyte protein 3, Protein VPreB3, VPREB3 |
| 计算分子量 | 123 aa, 14 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC020666 |
| 基因名称 | VPREB3 |
| Gene ID (NCBI) | 29802 |
| RRID | AB_2216942 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UKI3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPREB3 (formerly named 8HS20) is a protein that associates with mu chains in the endoplasmic reticulum of pre-B cell lines (PMID: 8491176; 22684248). VPREB3 is normally expressed by precursor B cells in bone marrow and by a subset of normal germinal center B cells in secondary lymphoid organs. It has been reported that VPREB3 expression is associated with B-cell tumors with c-MYC abnormalities (PMID: 20823132).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPREB3 antibody 11915-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
