×
Use Able AI chat for product recommendations

验证数据展示

  • IP experiment of SH-SY5Y using 31628-1-AP (same clone as 31628-1-PBS)

    IP experiment of SH-SY5Y using 31628-1-AP (same clone as 31628-1-PBS)

    IP result of anti-VLGR1 (IP:31628-1-AP, 4ug; Detection:31628-1-AP 1:500) with SH-SY5Y cells lysate 1920 ug. This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

  • WB analysis using 31628-1-AP (same clone as 31628-1-PBS)

    WB analysis using 31628-1-AP (same clone as 31628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

  • WB analysis using 31628-1-AP (same clone as 31628-1-PBS)

    WB analysis using 31628-1-AP (same clone as 31628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 31628-1-AP (same clone as 31628-1-PBS)

    IHC staining of human lung using 31628-1-AP (same clone as 31628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31628-1-AP (VLGR1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 31628-1-AP (same clone as 31628-1-PBS)

    IHC staining of human lung using 31628-1-AP (same clone as 31628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31628-1-AP (VLGR1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

VLGR1 Polyclonal antibody, PBS Only

VLGR1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 31628-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

ADGRV1, GPR98, ADGRV1 subunit alpha, ADGRV1 subunit beta, Adhesion G-protein coupled receptor V1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

31628-1-PBS targets VLGR1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34635

Product name: Recombinant human ADGRV1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2269-2812 aa of NM_032119

Sequence: ESIIVSLVYTEGGSRILPSSDTVRVNILANDNVAGIVSFQTASRSVIGHEGEILQFHVIRTFPGRGNVTVNWKIIGQNLELNFANFSGQLFFPEGSLNTTLFVHLLDDNIPEEKEVYQVILYDVRTQGVPPAGIALLDAQGYAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 98
别名 ADGRV1, GPR98, ADGRV1 subunit alpha, ADGRV1 subunit beta, Adhesion G-protein coupled receptor V1
计算分子量693 kDa
观测分子量217 kDa
GenBank蛋白编号NM_032119
基因名称 GPR98
Gene ID (NCBI) 84059
RRIDAB_3742457
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8WXG9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VLGR1 (very large G protein-coupled receptor-1), also known as ADGRV1, GPR98, and MASS, has a molecular weight of up to 700 kDa, making it the largest member of the 33 adhesive G protein-coupled receptors (ADGRs), a unique subfamily of the GPCR superfamily. The full-length VLGR1 encompasses ∼6300 amino acids and thus is difficult to analyze by electrophoresis. Vlgr1 is a core component of ankle-link complex in inner ear hair cells. Knock-out and mutation mouse models show that loss of Vlgr1 function leads to abnormal stereociliary development and hearing loss, indicating crucial roles of Vlgr1 in hearing transduction or auditory system development (PMID: 23180093). Western blot analysis detected VLGR1a isoform at an apparent molecular mass of 217 kDa .

||
New chat

Able™

正在加载，请稍候...