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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85097-1-RR (same clone as 85097-1-PBS)

    WB analysis using 85097-1-RR (same clone as 85097-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85097-1-RR (VLDLR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85097-1-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 85097-1-RR (same clone as 85097-1-PBS)

    FC experiment of THP-1 using 85097-1-RR (same clone as 85097-1-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug VLDLR Recombinant Antibody (85097-1-RR, Clone:242385H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85097-1-PBS in a different storage buffer formulation.

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VLDLR Recombinant monoclonal antibody, PBS Only

VLDLR Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 85097-1-PBS

产品说明书

CloneNo. 242385H3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

Very low-density lipoprotein receptor, VLDL receptor, VLDL-R

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85097-1-PBS targets VLDLR in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 very low density lipoprotein receptor
别名 Very low-density lipoprotein receptor, VLDL receptor, VLDL-R
计算分子量 96 kDa
观测分子量120 kDa
GenBank蛋白编号NM_003383
基因名称 VLDLR
Gene ID (NCBI) 7436
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP98155
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The very low-density lipoprotein receptor (VLDLR) is a member of the LDL receptor family with a complex molecular weight profile due to post-translational modifications. The calculated molecular weight of the core protein is approximately 84-86 kDa, however, VLDLR undergoes extensive glycosylation during processing, resulting in higher apparent molecular weights observed in SDS-PAGE analyses. Multiple studies report that glycosylated VLDLR migrates as broad bands between 90-180 kDa under reducing conditions, with the most commonly observed forms at approximately 96 kDa (core glycosylated form), 115-135 kDa (mature fully glycosylated form), and 150-180 kDa (hyperglycosylated or dimeric forms).