验证数据展示
经过测试的应用
| Positive WB detected in | HuH-7 cells, MCF-7 cells, MDA-MB-231 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19493-1-AP targets VLDLR in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, chicken, zebrafish |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | very low density lipoprotein receptor |
| 别名 | Very low-density lipoprotein receptor, VLDL receptor, VLDL-R |
| 计算分子量 | 96 kDa |
| 观测分子量 | 96 kDa |
| GenBank蛋白编号 | NM_003383 |
| 基因名称 | VLDLR |
| Gene ID (NCBI) | 7436 |
| RRID | AB_10644342 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P98155 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VLDLR binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. VLDLR binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (cytoplasmic domain of low-density lipoprotein receptor). For glycosylated, the MW of VLDLR migrates to ~160kd.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for VLDLR antibody 19493-1-AP | Download protocol |
| WB protocol for VLDLR antibody 19493-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning. | ||
Food Funct Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice | ||
Front Pharmacol Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. | ||
PLoS One Gut microbiota and butyrate contribute to nonalcoholic fatty liver disease in premenopause due to estrogen deficiency. | ||
Front Nutr Dietary camellia seed oil attenuates liver injury in mice chronically exposed to alcohol | ||
Front Oncol VLDLR disturbs quiescence of breast cancer stem cells in a ligand-independent function
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