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验证数据展示

  • WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

    WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 30166-1-AP (same clone as 30166-1-PBS)

    IHC staining of mouse kidney using 30166-1-AP (same clone as 30166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VIRMA/KIAA1429 antibody included
  • WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

    WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30166-1-AP (same clone as 30166-1-PBS)

    IF Staining of U2OS using 30166-1-AP (same clone as 30166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using VIRMA/KIAA1429 antibody (30166-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VIRMA/KIAA1429 Polyclonal antibody, PBS Only

VIRMA/KIAA1429 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30166-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA1429, VIRMA, MSTP054, virilizer

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VIRMA/KIAA1429 Ready-To-Use IHC Kit. VIRMA/KIAA1429 primary antibody included.

产品信息

30166-1-PBS targets VIRMA/KIAA1429 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32830

Product name: Recombinant human KIAA1429 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 786-1086 aa of BC003701

Sequence: FMRQILNSDTIGCCGDDNGLMEVEGAHTSRTMSINAAELKQLLQSKEESPENLFLELEKLVLEHSKDDDNLDSLLDSVVGLKQMLESSGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFTPFQAEEIDTDLDLVKVDLIELSEKCCSDFDLHSELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHVVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGIPPPKRPLKVSQKISSRGGFSGNRGGRGAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1429
别名 KIAA1429, VIRMA, MSTP054, virilizer
观测分子量200-210 kDa
GenBank蛋白编号BC003701
基因名称 KIAA1429
Gene ID (NCBI) 25962
RRIDAB_2935521
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ69YN4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274). Moreover, this antibody can not recognize short isoforms.

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