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验证数据展示

  • WB analysis of mouse small intestine using 14878-1-AP

    WB analysis of mouse small intestine using 14878-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14878-1-AP (VIPR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14878-1-AP

    IHC staining of human kidney using 14878-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 14878-1-AP

    IHC staining of human kidney using 14878-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VIPR1 Polyclonal antibody

VIPR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14878-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

HVR1, II, PACAP R 2, PACAP R2, PACAP type II receptor, RDC1, VAPC1, VIP R 1, VIPR, VIPR1, VIRG, VPAC1, VPCAP1R

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse small intestine tissue
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14878-1-AP targets VIPR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag6650

Product name: Recombinant human VIPR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-146 aa of BC064424

Sequence: AARLQEECDYVQMIEVQHKQCLEEAQLENETIGCSKMWDNLTCWPATPRGQVVVLACPLIFKLFSSIQGRNVSRSCTDEGWTHLEPGPYPIACGLDDKAASLDEQQTMFYGSVKTGY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vasoactive intestinal peptide receptor 1
别名 HVR1, II, PACAP R 2, PACAP R2, PACAP type II receptor, RDC1, VAPC1, VIP R 1, VIPR, VIPR1, VIRG, VPAC1, VPCAP1R
计算分子量 47 kDa
观测分子量50 kDa
GenBank蛋白编号BC064424
基因名称 VIPR1
Gene ID (NCBI) 7433
RRIDAB_2878089
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VIPR1 antibody 14878-1-APDownload protocol
IHC protocol for VIPR1 antibody 14878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

The p65-LOC727924-miR-26a/KPNA3-p65 regulatory loop mediates vasoactive intestinal peptide effects on osteoarthritis chondrocytes

Authors - Yusheng Li
View Article