验证数据展示

WB analysis of mouse kidney using 16488-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF Staining of mouse small intestine using 16488-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab, CoraLite® Plus 488 PIGR antibody (CL488-22024, green), CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).
IF Staining of mouse small intestine using 16488-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP experiment of mouse kidney using 16488-1-AP
IP result of anti-Villin (IP:16488-1-AP, 4ug; Detection:16488-1-AP 1:300) with mouse kidney tissue lysate 4000ug.
IHC staining of mouse small intestine using 16488-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16488-1-AP (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with VIL1 antibody included
IF Staining of COLO 320 using 16488-1-AP
Immunofluorescent analysis of COLO 320 cells using 16488-1-AP (Villin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 16488-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Villin (16488-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
IF Staining of mouse small intestine using 16488-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse small intestine using 16488-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO验证

Villin Polyclonal antibody

Villin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Cat No. 16488-1-AP

产品说明书

引用文献(19)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA and More (1)

别名

VIL1, D2S1471, VIL, villin 1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (19)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse kidney tissue, mouse colon tissue, mouse liver tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	mouse small intestine tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse small intestine tissue
Positive IF-Fro detected in	mouse small intestine tissue
Positive IF/ICC detected in	COLO 320 cells
Positive FC (Intra) detected in	HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy VIL1 Ready-To-Use IHC Kit. VIL1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 4 publications below
IF	See 12 publications below

产品信息

16488-1-AP targets Villin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, zebrafish
免疫原	CatNo: Ag9610 Product name: Recombinant human VIL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC017303 Sequence: MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWT 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	villin 1
别名	VIL1, D2S1471, VIL, villin 1
计算分子量	827aa,93 kDa; 826aa,93 kDa
观测分子量	93 kDa
GenBank蛋白编号	BC017303
基因名称	Villin
Gene ID (NCBI)	7429
RRID	AB_2215975
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P09327
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

实验方案

Product Specific Protocols
IF protocol for Villin antibody 16488-1-AP	Download protocol
IHC protocol for Villin antibody 16488-1-AP	Download protocol
IP protocol for Villin antibody 16488-1-AP	Download protocol
WB protocol for Villin antibody 16488-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
rat	IF	Sci Transl Med Oral delivery of liquid mRNA therapeutics by an engineered capsule for treatment of preclinical intestinal disease Authors - Xiangang Huang View Article
human	IF	Proc Natl Acad Sci U S A Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface. Authors - Jacqueline Corry View Article
mouse	IF	Clin Transl Med NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions. Authors - Zhixin Liu View Article
human	WB	mBio ASB3 expression aggravates inflammatory bowel disease by targeting TRAF6 protein stability and affecting the intestinal microbiota Authors - Mingyang Cheng View Article
pig	IF	PLoS Pathog Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids. Authors - Qi-Yue Yang View Article
mouse	WB	Front Cell Dev Biol Cathelicidin-WA Protects Against LPS-Induced Gut Damage Through Enhancing Survival and Function of Intestinal Stem Cells. Authors - Sisi Wang View Article

验证数据展示

WB analysis of mouse kidney using 16488-1-AP

IF Staining of mouse small intestine using 16488-1-AP

IF Staining of mouse small intestine using 16488-1-AP

IP experiment of mouse kidney using 16488-1-AP

IHC staining of mouse small intestine using 16488-1-AP

IF Staining of COLO 320 using 16488-1-AP

FC experiment of HepG2 using 16488-1-AP

IF Staining of mouse small intestine using 16488-1-AP

IF Staining of mouse small intestine using 16488-1-AP

Villin Polyclonal antibody

Villin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Oral delivery of liquid mRNA therapeutics by an engineered capsule for treatment of preclinical intestinal disease

Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.

NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.

ASB3 expression aggravates inflammatory bowel disease by targeting TRAF6 protein stability and affecting the intestinal microbiota

Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids.

Cathelicidin-WA Protects Against LPS-Induced Gut Damage Through Enhancing Survival and Function of Intestinal Stem Cells.