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验证数据展示

  • WB analysis using 25261-1-AP

    WB analysis using 25261-1-AP

    unboiled rat brain tissue were subjected to SDS PAGE followed by western blot with 25261-1-AP (VGLUT2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of fetal human brain using 25261-1-AP

    WB analysis of fetal human brain using 25261-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25261-1-AP (VGLUT2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

VGLUT2 Polyclonal antibody

VGLUT2 Polyclonal Antibody for WB, ELISA
Cat No. 25261-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

SLC17A6, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inunboiled rat brain tissue, fetal human brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25261-1-AP targets VGLUT2 in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
别名 SLC17A6, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI
计算分子量 64 kDa
观测分子量 60 kDa
GenBank蛋白编号NM_020346
基因名称 VGLUT2
Gene ID (NCBI) 57084
RRIDAB_2879992
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2U8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicle (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).

实验方案

Product Specific Protocols
WB protocol for VGLUT2 antibody 25261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols