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验证数据展示

  • WB analysis using 29469-1-AP (same clone as 29469-1-PBS)

    WB analysis using 29469-1-AP (same clone as 29469-1-PBS)

    Various tissue were subjected to SDS PAGE followed by western blot with 29469-1-AP (VGLUT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29469-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 29469-1-AP (same clone as 29469-1-PBS)

    IHC staining of mouse brain using 29469-1-AP (same clone as 29469-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29469-1-AP (VGLUT1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29469-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VGLUT1 antibody included

VGLUT1 Polyclonal antibody, PBS Only

VGLUT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29469-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

1, SLC17A7, BNPI, Brain-specific Na(+)-dependent inorganic phosphate cotransporter

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® VGLUT1 Ready-To-Use IHC Kit. VGLUT1 primary antibody included.

产品信息

29469-1-PBS targets VGLUT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31193

Product name: Recombinant human VGLUT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 500-560 aa of NM_020309

Sequence: PEEMSEEKCGFVGHDQLAGSDDSEMEDEAEPPGAPPAPPPSYGATHSTFQPPRPPPPVRDY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
别名 1, SLC17A7, BNPI, Brain-specific Na(+)-dependent inorganic phosphate cotransporter
计算分子量 62 kDa
观测分子量55-60 kDa
GenBank蛋白编号NM_020309
基因名称 VGLUT1
Gene ID (NCBI) 57030
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2U7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vesicular glutamate transporter 1 (VGLUT1) is a critical protein involved in the packaging and release of the neurotransmitter glutamate in synaptic vesicles. VGLUT1 is predominantly expressed in neurons that release glutamate, the primary excitatory neurotransmitter in the central nervous system, it is a marker of glutamatergic neurons. VGLUT1 has several isoforms with the moleculat weight range from 53-61 kDa.

VGLUT1 is a multiple transmembrane protein, which is easily to aggregate if the sample is bolied or treated with high temperature, thus we recommend to use unboiled sample for the detection.

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