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验证数据展示

  • WB analysis using 10124-2-AP (same clone as 10124-2-PBS)

    WB analysis using 10124-2-AP (same clone as 10124-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10124-2-AP (VGLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10124-2-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10124-2-AP (same clone as 10124-2-PBS)

    IHC staining of human prostate cancer using 10124-2-AP (same clone as 10124-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10124-2-AP (VGLL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10124-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VGLL1 Polyclonal antibody, PBS Only

VGLL1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10124-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10124-2-PBS targets VGLL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0169

Product name: Recombinant human VGLL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 38-255 aa of BC000045

Sequence: SSVVDEHFSRALSNIKSPQELTPSSQSEGVMLKNDDSMSPNQWRYSSPWTKPQPEVPVTNRAANCNLHVPGPMAVNQFSPSLARRASVRPGELWHFSSLAGTSSLEPGYSHPFPARHLVPEPQPDGKREPLLSLLQQDRCLARPQESAARENGNPGQIAGSTGLLFNLPPGSVHYKKLYVSRGSASTSLPNETLSELETPGKYSLTPPNHWGHPHRYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vestigial like 1 (Drosophila)
别名
计算分子量 29 kDa
观测分子量 26-29 kDa
GenBank蛋白编号BC000045
基因名称 VGLL1
Gene ID (NCBI) 51442
RRIDAB_2218174
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99990
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VGLL1 is a specific coactivator for the mammalian TEFs. It can bind proteins of the TEA domain family of transcription factors. Vgll1-TEFs complex upregulates the expression of IGFBP-5, a proliferation-promoting gene, and facilitates anchorage-independent cell proliferation. The calculated molecular weight of VGLL1 is 29 kDa, and the observed molecular weight is 26-29 kDa(PMID:31816819,33087697).

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