验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IF/ICC detected in | bEnd.3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87120-3-RR targets VEGFR3/FLT4 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg6355 Product name: Recombinant Mouse Flt-4 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 25-775 aa of NM_008029.3 Sequence: YSMTPPTLNITEDSYVIDTGDSLSISCRGQHPLEWTWPGAQEVLTTGGKDSEDTRVVHDCEGTEARPYCKVLLLAQTHANNTGSYHCYYKYIKARIEGTTAASTYVFVRDFKHPFINKPDTLLVNRKDSMWVPCLVSIPGLNITLRSQSSALHPDGQEVLWDDRRGMRVPTQLLRDALYLQCETTWGDQNFLSNLFVVHITGNELYDIQLYPKKSMELLVGEKLVLNCTVWAEFDSGVTFDWDYPGKQAERAKWVPERRSQQTHTELSSILTIHNVSQNDLGPYVCEANNGIQRFRESTEVIVHEKPFISVEWLKGPVLEATAGDELVKLPVKLAAYPPPEFQWYKDRKAVTGRHNPHALVLKEVTEASAGVYTLALWNSAAGLRQNISLELVVNVPPHIHEKEASSPSIYSRHSRQTLTCTAYGVPQPLSVQWHWRPWTPCKTFAQRSLRRRQQRDGMPQCRDWKEVTTQDAVNPIESLDSWTEFVEGKNKTVSKLVIQDANVSAMYKCVVVNKVGQDERLIYFYVTTIPDGFSIESEPSEDPLEGQSVRLSCRADNYTYEHLRWYRLNLSTLHDAQGNPLLLDCKNVHLFATPLEANLEEAEPGARHATLSLNIPRVAPEDEGDYVCEVQDRRSQDKHCHKKYLSVQALEAPRLTQNLTDLLVNVSDSLEMRCPVAGAHVPSIVWYKDERLLEKESGIDLADSNQRLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSEDKGSME 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | FMS-like tyrosine kinase 4 |
| 别名 | Flt4, Flt-4, Fms-like tyrosine kinase 4, VEGFR-3 |
| 计算分子量 | 153 kDa |
| 观测分子量 | 153 kDa |
| GenBank蛋白编号 | NM_008029.3 |
| 基因名称 | Flt4 |
| Gene ID (NCBI) | 14257 |
| RRID | AB_3745353 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35917 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FLT4, also named VEGFR-3, belongs to the protein kinase superfamily, Tyr protein kinase family, and CSF-1/PDGF receptor subfamily. It is a receptor for VEGFC. FLT4 has a tyrosine-protein kinase activity. Defects in FLT4 cause lymphedema hereditary type 1 (LYH1A). Defects in FLT4 are found in juvenile hemangioma.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for VEGFR3/FLT4 antibody 87120-3-RR | Download protocol |
| WB protocol for VEGFR3/FLT4 antibody 87120-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
