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验证数据展示

  • WB analysis of mouse lung using 83366-1-RR (same clone as 83366-1-PBS)

    WB analysis of mouse lung using 83366-1-RR (same clone as 83366-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83366-1-RR (VEGFR2/KDR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83366-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00394-1

    Cytometric bead array standard curve of MP00394-1

    Cytometric bead array standard curve of MP00394-1, MOUSE VEGFR2/KDR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83366-1-PBS. Detection antibody: 83366-3-PBS. Standard: Eg0703. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00394-3

    Cytometric bead array standard curve of MP00394-3

    Cytometric bead array standard curve of MP00394-3, MOUSE VEGFR2/KDR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83366-1-PBS. Detection antibody: 83366-2-PBS. Standard: Eg0703. Range: 5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83366-1-RR

    Affinity and Kinetic Characterization of 83366-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83366-1-RR against Mouse VEGFR2/KDR were performed. The affinity constant is 1.88 nM.

Mouse VEGFR2/KDR Recombinant antibody, PBS Only (Capture)

VEGFR2/KDR Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83366-1-PBS

产品说明书

CloneNo. 240214D7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

VEGFR-2/Kdr, Kdr, Krd 1, Flk1, Flk 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83366-1-PBS targets VEGFR2/KDR as part of a matched antibody pair:

MP00394-1: 83366-1-PBS capture and 83366-3-PBS detection (validated in Cytometric bead array)

MP00394-3: 83366-1-PBS capture and 83366-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinase insert domain protein receptor
别名 VEGFR-2/Kdr, Kdr, Krd 1, Flk1, Flk 1
计算分子量150KD
观测分子量150-190 kDa
GenBank蛋白编号NM_010612.2
基因名称 VEGFR-2
Gene ID (NCBI) 16542
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35918-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VEGFR2, also named CD309, KDR, and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In the case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis, and sprouting.