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验证数据展示

  • WB analysis using 30323-1-AP

    WB analysis using 30323-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30323-1-AP (VEGFD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 30323-1-AP

    IHC staining of mouse lung using 30323-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30323-1-AP (VEGFD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 30323-1-AP

    IHC staining of mouse lung using 30323-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30323-1-AP (VEGFD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VEGFD Polyclonal antibody

VEGFD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30323-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Figf, c fos induced growth factor, Vascular endothelial growth factor D, VEGF D, VEGF-D

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30323-1-AP targets VEGFD in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 c-fos induced growth factor
别名 Figf, c fos induced growth factor, Vascular endothelial growth factor D, VEGF D, VEGF-D
计算分子量41 kDa
观测分子量38-40 kDa
GenBank蛋白编号NM_010216
基因名称 Figf
Gene ID (NCBI) 14205
RRIDAB_3742335
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP97946
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFD is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.

实验方案

Product Specific Protocols
IHC protocol for VEGFD antibody 30323-1-APDownload protocol
WB protocol for VEGFD antibody 30323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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