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验证数据展示

  • WB analysis of MCF-7 using 67116-1-Ig

    WB analysis of MCF-7 using 67116-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 67116-1-Ig

    IF Staining of MCF-7 using 67116-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using VEGFC antibody (67116-1-Ig, Clone: 2B11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

VEGF-C Monoclonal antibody

VEGF-C Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67116-1-Ig

产品说明书

CloneNo. 2B11F7
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

VEGFC, VEGF C, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C, 2B11F7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells, NCI-H1299 cells, A549 cells, LNCaP cells, HT-1080 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFC Ready-To-Use IHC Kit. VEGFC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67116-1-Ig targets VEGF-C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 VEGF-C fusion protein Ag18182 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vascular endothelial growth factor C
别名 VEGFC, VEGF C, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C, 2B11F7
计算分子量 419 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC035212
基因名称 VEGFC
Gene ID (NCBI) 7424
RRIDAB_2882420
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49767
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VEGF-C antibody 67116-1-IgDownload protocol
IF protocol for VEGF-C antibody 67116-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Cycle

CircHGS enhances the progression of bladder cancer by regulating the miR-513a-5p/VEGFC axis and activating the AKT/mTOR signaling pathway

Authors - Yi Zhu
View Article