验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, RAW 264.7 cells, HCT 116 cells, SW480 cells |
| Positive IHC detected in | human prostate cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22601-1-AP targets VEGF-C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18182 Product name: Recombinant human VEGFC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 179-228 aa of BC035212 Sequence: SYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRRS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vascular endothelial growth factor C |
| 别名 | VEGFC, VEGF C, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C |
| 计算分子量 | 419 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC035212 |
| 基因名称 | VEGFC |
| Gene ID (NCBI) | 7424 |
| RRID | AB_2879132 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49767 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for VEGF-C antibody 22601-1-AP | Download protocol |
| IHC protocol for VEGF-C antibody 22601-1-AP | Download protocol |
| WB protocol for VEGF-C antibody 22601-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination. | ||
Clin Transl Med VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis. | ||
Cancer Lett The Hippo-TAZ axis mediates vascular endothelial growth factor C in glioblastoma-derived exosomes to promote angiogenesis. | ||
Oxid Med Cell Longev Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms. | ||
Front Pharmacol Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway |
