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验证数据展示

  • WB analysis using 84529-4-RR

    WB analysis using 84529-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84529-4-RR (VE-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84529-4-RR

    Affinity and Kinetic Characterization of 84529-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84529-4-RR against Mouse VE-cadherin/CD144 were performed. The affinity constant is 51.6 nM.

VE-cadherin/CD144 Recombinant antibody

VE-cadherin/CD144 Recombinant Antibody for WB, ELISA
Cat No. 84529-4-RR

产品说明书

CloneNo. 241953A12

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, ELISA

别名

VE-cadherin, VE Cad, Cdh5, Cd144, cadherin 5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inbEnd.3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84529-4-RR targets VE-cadherin/CD144 in WB, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 5
别名 VE-cadherin, VE Cad, Cdh5, Cd144, cadherin 5
计算分子量88kDa
观测分子量125-130 kDa, 90-100 kDa
GenBank蛋白编号NM_009868.4
基因名称 Cdh5
Gene ID (NCBI) 12562
RRIDAB_3672038
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP55284
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

实验方案

Product Specific Protocols
WB protocol for VE-cadherin/CD144 antibody 84529-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols