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验证数据展示

  • IF Staining of bEnd.3 using 84529-3-RR

    IF Staining of bEnd.3 using 84529-3-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using VE-cadherin antibody (84529-3-RR, Clone: 241953H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse spleen using 84529-3-RR

    IHC staining of mouse spleen using 84529-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84529-3-RR (VE-cadherin/CD144 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 84529-3-RR

    IHC staining of mouse spleen using 84529-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84529-3-RR (VE-cadherin/CD144 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84529-3-RR

    Affinity and Kinetic Characterization of 84529-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84529-3-RR against Mouse VE-cadherin were performed. The affinity constant is 1.22 nM.

VE-cadherin/CD144 Recombinant antibody

VE-cadherin/CD144 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84529-3-RR

产品说明书

CloneNo. 241953H6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, IF/ICC, ELISA

别名

VE-cadherin, 241953H6, 7B4, cadherin 5, Cd144

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inbEnd.3 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84529-3-RR targets VE-cadherin/CD144 in IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 VE-cadherin/CD144 fusion protein Eg0921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 5
别名 VE-cadherin, 241953H6, 7B4, cadherin 5, Cd144
计算分子量88kDa
GenBank蛋白编号NM_009868.4
基因名称 Cdh5
Gene ID (NCBI) 12562
RRIDAB_3672037
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55284
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).

实验方案

Product Specific Protocols
IHC protocol for VE-cadherin/CD144 antibody 84529-3-RRDownload protocol
IF protocol for VE-cadherin/CD144 antibody 84529-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols