验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse brain tissue, HuH-7 cells |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy VDAC2 Ready-To-Use IHC Kit. VDAC2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
55261-1-AP targets VDAC2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | voltage-dependent anion channel 2 |
| 别名 | FLJ23841, hVDAC2, VDAC 2, VDAC-2 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 31-33 kDa |
| GenBank蛋白编号 | NM_003375 |
| 基因名称 | VDAC2 |
| Gene ID (NCBI) | 7417 |
| RRID | AB_10852961 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P45880 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for VDAC2 antibody 55261-1-AP | Download protocol |
| IHC protocol for VDAC2 antibody 55261-1-AP | Download protocol |
| WB protocol for VDAC2 antibody 55261-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Physiol Biochem Knockout of VDAC1 in H9c2 Cells Promotes Oxidative Stress-Induced Cell Apoptosis through Decreased Mitochondrial Hexokinase II Binding and Enhanced Glycolytic Stress. | ||
J Mol Med (Berl) Cerium oxide nanoparticles as potent inhibitors of ferroptosis: role of antioxidant activity and protein regulation |
