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VCPIP1 Polyclonal antibody

VCPIP1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17802-1-AP

Print datasheet

Synonyms

DUBA3, KIAA1850, VCIP135, VCPIP1

验证数据展示

  • IHC staining of human ovary tumor using 17802-1-AP

    IHC staining of human ovary tumor using 17802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17802-1-AP (VCPIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of MCF-7 using 17802-1-AP

    WB analysis of MCF-7 using 17802-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17802-1-AP (VCPIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17802-1-AP

    IHC staining of human breast cancer using 17802-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17802-1-AP (VCPIP1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inMCF7 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17802-1-AP is VCPIP1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen VCPIP1 fusion protein Ag12035 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name valosin containing protein (p97)/p47 complex interacting protein 1
Synonyms DUBA3, KIAA1850, VCIP135, VCPIP1
Calculated Molecular Weight 1222 aa, 134 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC094799
Gene Symbol VCPIP1
Gene ID (NCBI) 80124
RRIDAB_10699873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JH7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VCPIP1 antibody 17802-1-APDownload protocol
IHC protocol for VCPIP1 antibody 17802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Immunol Res

USP22 deubiquitinates CD274 to suppress anti-cancer immunity.

Authors - Xing Huang
View Article
humanRIP

Genes Dis

Downregulation of nc886 contributes to prostate cancer cell invasion and TGFβ1-induced EMT.

Authors - Ronghui Yang
View Article