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验证数据展示

  • WB analysis of HUVEC using 84249-4-RR

    WB analysis of HUVEC using 84249-4-RR

    TNF alpha treated HUVEC cells were subjected to SDS PAGE followed by western blot with 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84249-4-RR

    IHC staining of human tonsillitis using 84249-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VCAM-1 antibody included
  • IHC staining of human tonsillitis using 84249-4-RR

    IHC staining of human tonsillitis using 84249-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VCAM-1 antibody included
  • IF Staining of human tonsillitis using 84249-4-RR

    IF Staining of human tonsillitis using 84249-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VCAM-1/CD106 antibody (84249-4-RR, Clone: 241284B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 84249-4-RR

    IF Staining of human tonsillitis using 84249-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VCAM-1/CD106 antibody (84249-4-RR, Clone: 241284B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84249-4-RR

    Affinity and Kinetic Characterization of 84249-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84249-4-RR against Human VCAM-1/CD106 were performed. The affinity constant is 2.18 nM.

VCAM-1/CD106 Recombinant monoclonal antibody

VCAM-1/CD106 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84249-4-RR

产品说明书

CloneNo. 241284B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

VCAM1, VCAM-1, 241284B2, CD106, INCAM 100

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTNF alpha treated HUVEC cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy VCAM-1 Ready-To-Use IHC Kit. VCAM-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84249-4-RR targets VCAM-1/CD106 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular cell adhesion molecule 1
别名 VCAM1, VCAM-1, 241284B2, CD106, INCAM 100
计算分子量81 kDa
观测分子量100 kDa
GenBank蛋白编号NM_001078.4
基因名称 VCAM-1
Gene ID (NCBI) 7412
RRIDAB_3671799
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19320-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

实验方案

Product Specific Protocols
IF protocol for VCAM-1/CD106 antibody 84249-4-RRDownload protocol
IHC protocol for VCAM-1/CD106 antibody 84249-4-RRDownload protocol
WB protocol for VCAM-1/CD106 antibody 84249-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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