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验证数据展示

  • WB analysis of HUVEC using 84249-4-RR (same clone as 84249-4-PBS)

    WB analysis of HUVEC using 84249-4-RR (same clone as 84249-4-PBS)

    TNF alpha treated HUVEC cells were subjected to SDS PAGE followed by western blot with 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VCAM-1 antibody included
  • IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VCAM-1 antibody included
  • Sandwich ELISA standard curve of MP01145-3

    Sandwich ELISA standard curve of MP01145-3

    Sandwich ELISA standard curve of MP01145-3, Human VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 84249-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02345. 84249-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP01145-3

    Sample test of MP01145-3

    Serum of six individual healthy human donors was measured. The human VCAM-1/CD106 concentration of detected samples was determined to be 188.79 ng/mL with a range of 126.68 - 336.06 ng/mL.

  • IF Staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    IF Staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VCAM-1/CD106 antibody (84249-4-RR, Clone: 241284B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    IF Staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VCAM-1/CD106 antibody (84249-4-RR, Clone: 241284B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01145-3

    Cytometric bead array standard curve of MP01145-3

    Cytometric bead array standard curve of MP01145-3, VCAM-1/CD106 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84249-4-PBS. Detection antibody: 84249-2-PBS. Standard: RP02345. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 84249-4-RR

    Affinity and Kinetic Characterization of 84249-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84249-4-RR against Human VCAM-1/CD106 were performed. The affinity constant is 2.18 nM.

VCAM-1/CD106 Recombinant antibody, PBS Only (Capture)

VCAM-1/CD106 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84249-4-PBS

产品说明书

CloneNo. 241284B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

VCAM1, VCAM-1, 241284B2, CD106, INCAM 100

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VCAM-1 Ready-To-Use IHC Kit. VCAM-1 primary antibody included.

产品信息

84249-4-PBS targets VCAM-1/CD106 as part of a matched antibody pair:

MP01145-3: 84249-4-PBS capture and 84249-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular cell adhesion molecule 1
别名 VCAM1, VCAM-1, 241284B2, CD106, INCAM 100
计算分子量81 kDa
观测分子量100 kDa
GenBank蛋白编号NM_001078.4
基因名称 VCAM-1
Gene ID (NCBI) 7412
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19320-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).