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VCAM-1 Polyclonal antibody

VCAM-1 Polyclonal Antibody for WB, IF-P, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, IF-P, IHC, ELISA

Conjugate

Unconjugated

Cat No : 30958-1-AP

Print datasheet

Synonyms

CD106, Vcam 1, Vcam1

验证数据展示

  • WB analysis using 30958-1-AP

    WB analysis using 30958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30958-1-AP (VCAM-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 30958-1-AP

    IHC staining of mouse spleen using 30958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 30958-1-AP

    IHC staining of mouse spleen using 30958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 30958-1-AP

    IF Staining of mouse spleen using 30958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using VCAM-1 antibody (30958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 30958-1-AP

    IF Staining of mouse spleen using 30958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using VCAM-1 antibody (30958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat spleen tissue, mouse spleen tissue, C2C12 cells
Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30958-1-AP targets VCAM-1 in WB, IF, IHC, ELISA applications and shows reactivity with Mouse, Rat samples.

Tested Applications WB, IF-P, IHC, ELISA
Tested Reactivity Mouse, Rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vascular cell adhesion molecule 1
Synonyms CD106, Vcam 1, Vcam1
Calculated Molecular Weight81KD
Observed Molecular Weight100-110 kDa
GenBank Accession NumberCAA47989.1
Gene Symbol Vcam1
Gene ID (NCBI) 22329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

实验方案

Product Specific Protocols
WB protocol for VCAM-1 antibody 30958-1-APDownload protocol
IHC protocol for VCAM-1 antibody 30958-1-APDownload protocol
IF protocol for VCAM-1 antibody 30958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols