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VCAM-1/CD106 Polyclonal antibody

VCAM-1/CD106 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11444-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

VCAM1, VCAM-1, V CAM 1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inTNF alpha treated HUVEC cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human spleen tissue, human placenta tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VCAM-1 Ready-To-Use IHC Kit. VCAM-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11444-1-AP targets VCAM-1/CD106 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 VCAM-1/CD106 fusion protein Ag1993 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vascular cell adhesion molecule 1
别名 VCAM1, VCAM-1, V CAM 1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM
计算分子量 739 aa, 81 kDa
观测分子量110 kDa
GenBank蛋白编号BC017276
基因名称 VCAM-1
Gene ID (NCBI) 7412
RRIDAB_2214232
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19320
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

实验方案

Product Specific Protocols
WB protocol for VCAM-1/CD106 antibody 11444-1-APDownload protocol
IHC protocol for VCAM-1/CD106 antibody 11444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Cardiovasc Res

HDAC4 regulates vascular inflammation via activation of autophagy.

Authors - Di Yang
humanIF

EBioMedicine

Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion.

Authors - Peng Zhou
IHC

Aging Dis

Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.

Authors - Danyang Li

ACS Appl Mater Interfaces

Inflammation-Driven Nanohitchhiker Enhances Postoperative Immunotherapy by Alleviating Prostaglandin E2-Mediated Immunosuppression

Authors - Yingke Liu
IF

Br J Pharmacol

Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation

Authors - Hang Su
human

Br J Pharmacol

Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.

Authors - Chuanrui Ma
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