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验证数据展示

  • WB analysis of fetal human brain using 67108-1-Ig

    WB analysis of fetal human brain using 67108-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67108-1-Ig

    IF Staining of A431 using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of HUVEC using 67108-1-Ig

    IF Staining of HUVEC using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

VAV2 Monoclonal antibody

VAV2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67108-1-Ig

产品说明书

CloneNo. 3F11B5

宿主/亚型

Mouse / IgG2a

种属反应性

Human, pig, mouse, rat

应用

WB, IF/ICC, ELISA

别名

VAV 2, VAV2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected infetal human brain tissue, A431 cells, HeLa cells, HEK-293 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inA431 cells, HeLa cells, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy VAV2 Ready-To-Use IHC Kit. VAV2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67108-1-Ig targets VAV2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, pig, mouse, rat
免疫原

CatNo: Ag17988

Product name: Recombinant human VAV2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 98-326 aa of BC132967

Sequence: DLFDVRDFGKVISAVSRLSLHSIAQNKGIRPFPSEETTENDDDVYRSLEELADEHDLGEDIYDCVPCEDGGDDIYEDIIKVEVQQPMKMGMTEDDKRNCCLLEIQETEAKYYRTLEDIEKNYMSPLRLVLSPADMAAVFINLEDLIKVHHSFLRAIDVSVMVGGSTLAKVFLDFKERLLIYGEYCSHMEHAQNTLNQLLASREDFRQKVEECTLKVQDGKFKLQDLLVV

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 vav 2 guanine nucleotide exchange factor
别名 VAV 2, VAV2
计算分子量 839 aa, 97 kDa
观测分子量 101 kDa
GenBank蛋白编号BC132967
基因名称 VAV2
Gene ID (NCBI) 7410
RRIDAB_2882412
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP52735
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for VAV2 antibody 67108-1-IgDownload protocol
WB protocol for VAV2 antibody 67108-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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