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验证数据展示

  • WB analysis using 86933-2-RR

    WB analysis using 86933-2-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 86933-2-RR (VAV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 86933-2-RR

    IF Staining of Jurkat using 86933-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using VAV1 antibody (86933-2-RR, Clone: 251870A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86933-2-RR

    Affinity and Kinetic Characterization of 86933-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86933-2-RR against Human VAV1 were performed. The affinity constant is below 1 pM.

VAV1 Recombinant monoclonal antibody

VAV1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86933-2-RR

产品说明书

CloneNo. 251870A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Proto oncogene vav, Proto-oncogene vav, VAV

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, THP-1 cells, K-562 cells, Raji cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86933-2-RR targets VAV1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38665

Product name: Recombinant human VAV1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 442-790 aa of BC013361

Sequence: EQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCHRCRASAHKECLGRVPPCGRHGQDFPGTMKKDKLHRRAQDKKRNELGLPKMEVFQEYYGLPPPPGAIGPFLRLNPGDIVELTKAEAEQNWWEGRNTSTNEIGWFPCNRVKPYVHGPPQDLSVHLWYAGPMERAGAESILANRSDGTFLVRQRVKDAAEFAISIKYNVEVKHIKIMTAEGLYRITEKKAFRGLTELVEFYQQNSLKDCFKSLDTTLQFPFKEPEKRTISRPAVGSTKYFGTAKARYDFCARDRSELSLKEGDIIKILNKKGQQGWWRGEIYGRVGWFPANYVEEDYSEYC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vav 1 guanine nucleotide exchange factor
别名 Proto oncogene vav, Proto-oncogene vav, VAV
计算分子量 845 aa, 98 kDa
观测分子量 98 kDa
GenBank蛋白编号BC013361
基因名称 VAV1
Gene ID (NCBI) 7409
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15498
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vav proteins mainly act as enzymes that catalyze the activation step of Rho subfamily GTPases during cell signaling. There are three family members:VAV1, VAV2 and VAV3. Vav1 is specifically expressed in the hematopoietic system, whereas Vav2 and Vav3 are more ubiquitously expressed (PMID:14607270). Vav1 is physiologically active as a GDP/GTP nucleotide exchange factor (GEF) in the hematopoietic system (PMID:31654719).

实验方案

Product Specific Protocols
IF protocol for VAV1 antibody 86933-2-RRDownload protocol
WB protocol for VAV1 antibody 86933-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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