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验证数据展示

  • WB analysis using 86847-1-RR (same clone as 86847-1-PBS)

    WB analysis using 86847-1-RR (same clone as 86847-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86847-1-RR (VAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 86847-1-RR (same clone as 86847-1-PBS)

    IF Staining of SH-SY5Y using 86847-1-RR (same clone as 86847-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using VAT1 antibody (86847-1-RR, Clone: 251841D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86847-1-RR (same clone as 86847-1-PBS)

    IHC staining of mouse brain using 86847-1-RR (same clone as 86847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86847-1-RR (VAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VAT1 antibody included
  • IHC staining of mouse brain using 86847-1-RR (same clone as 86847-1-PBS)

    IHC staining of mouse brain using 86847-1-RR (same clone as 86847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86847-1-RR (VAT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VAT1 antibody included

VAT1 Recombinant monoclonal antibody, PBS Only

VAT1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86847-1-PBS

产品说明书

CloneNo. 251841D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:1.-.-.-, Synaptic vesicle membrane protein VAT-1 homolog, VATI

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VAT1 Ready-To-Use IHC Kit. VAT1 primary antibody included.

产品信息

86847-1-PBS targets VAT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16652

Product name: Recombinant human VAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-393 aa of BC015041

Sequence: MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vesicle amine transport protein 1 homolog (T. californica)
别名 EC:1.-.-.-, Synaptic vesicle membrane protein VAT-1 homolog, VATI
计算分子量 42 kDa
观测分子量 45 kDa
GenBank蛋白编号BC015041
基因名称 VAT1
Gene ID (NCBI) 10493
RRIDAB_3745159
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99536
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vesicle amine transport protein 1 (VAT1) is expressed in neurons and neuroendocrine cells, where it participates in vesicular monoamine uptake and in the docking/fusion steps of neurotransmitter release. Beyond the nervous system, VAT1 binds anionic phospholipids (phosphatidic acid and phosphatidylserine) and catalyses their inter-membrane transfer, thereby integrating lipid metabolism with vesicle trafficking. Recent tumour-biology studies have revealed that VAT1 is frequently up-regulated in aggressive gliomas and gastric cancers; its abundance correlates with enhanced cell migration, radioresistance and an immunosuppressive micro-environment. (PMID: 39832459,PMID: 31524269)

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