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验证数据展示

  • WB analysis of HepG2 using 67474-1-Ig

    WB analysis of HepG2 using 67474-1-Ig

    WB result of VAT1 antibody (67474-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAT1 transfected HepG2 cells.

  • WB analysis using 67474-1-Ig

    WB analysis using 67474-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67474-1-Ig (VAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 67474-1-Ig

    IHC staining of mouse pancreas using 67474-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67474-1-Ig (VAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAT1 antibody included
  • Featured Product
  • KD/KO验证

VAT1 Monoclonal antibody

VAT1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67474-1-Ig

产品说明书

CloneNo. 2C8A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

VATI, Synaptic vesicle membrane protein VAT-1 homolog, EC:1.-.-.-, 2C8A7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHSC-T6 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VAT1 Ready-To-Use IHC Kit. VAT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67474-1-Ig targets VAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 VAT1 fusion protein Ag29812 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vesicle amine transport protein 1 homolog (T. californica)
别名 VATI, Synaptic vesicle membrane protein VAT-1 homolog, EC:1.-.-.-, 2C8A7
计算分子量 42 kDa
观测分子量 42-50 kDa
GenBank蛋白编号BC015041
基因名称 VAT1
Gene ID (NCBI) 10493
RRIDAB_2882701
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ99536
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VAT1 antibody 67474-1-IgDownload protocol
IHC protocol for VAT1 antibody 67474-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols