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验证数据展示

  • WB analysis of HepG2 using 67474-1-Ig

    WB analysis of HepG2 using 67474-1-Ig

    WB result of VAT1 antibody (67474-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAT1 transfected HepG2 cells.

  • WB analysis using 67474-1-Ig

    WB analysis using 67474-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67474-1-Ig (VAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 67474-1-Ig

    IHC staining of mouse pancreas using 67474-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67474-1-Ig (VAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAT1 antibody included
  • Featured Product
  • KD/KO验证

VAT1 Monoclonal antibody

VAT1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67474-1-Ig

产品说明书

CloneNo. 2C8A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

VATI, Synaptic vesicle membrane protein VAT-1 homolog, EC:1.-.-.-, 2C8A7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHSC-T6 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VAT1 Ready-To-Use IHC Kit. VAT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67474-1-Ig targets VAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag29812

Product name: Recombinant human VAT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-393 aa of BC015041

Sequence: MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vesicle amine transport protein 1 homolog (T. californica)
别名 VATI, Synaptic vesicle membrane protein VAT-1 homolog, EC:1.-.-.-, 2C8A7
计算分子量 42 kDa
观测分子量 42-50 kDa
GenBank蛋白编号BC015041
基因名称 VAT1
Gene ID (NCBI) 10493
RRIDAB_2882701
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ99536
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VAT1 antibody 67474-1-IgDownload protocol
IHC protocol for VAT1 antibody 67474-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols