验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, HeLa cells, MCF-7 cells |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12730-1-AP targets VASH1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | mouse, zebrafish |
| 免疫原 |
CatNo: Ag3435 Product name: Recombinant human VASH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC009031 Sequence: MPGGKKVAGGGSSGATPTSAAATAPSGVRRLETSEGTSAQRDEEPEEEGEEDLRDGGVPFFVNRGGLPVDEATWERMWKHVAKIHPDGEKVAQRIRGATDLPKIPIPSVPTFQPSTPVPERLEAVQRYIRELQYNHTGTQFFEIKKSRPLTGLMDLAKEMTKEALPIKCLEAVILGMYPSSPEGEGSGLLWASASCSESEGGVG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vasohibin 1 |
| 别名 | Tyrosine carboxypeptidase 1, Tubulinyl-Tyr carboxypeptidase 1, Tubulin carboxypeptidase 1, TTCP 1, KIAA1036 |
| 计算分子量 | 365 aa, 41 kDa |
| 观测分子量 | 36 and 41 kDa |
| GenBank蛋白编号 | BC009031 |
| 基因名称 | VASH1 |
| Gene ID (NCBI) | 22846 |
| RRID | AB_2288318 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7L8A9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VASH1, also named as vasohibin 1, is a angiogenesis inhibitor induced by the stimulation with VEGF. High expression of VASH1 has been researched in some tumors as a result of VEGF induction. It has been suggested that VASH-1 is clinically significant in bladder cancer, colorectal cancer and NSCLC. VASH-1 may be a potential marker of gastric cancer and a target for vascular targeting therapy. 12730-1-AP detects 41 kDa form secreted protein and 36 kDa form protein accumulated within the cells or pericellular milieu in SDS-PAGE.(PMID:19057892, 29042936, 29552154, 26666821)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VASH1 antibody 12730-1-AP | Download protocol |
| IF protocol for VASH1 antibody 12730-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Development Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk.
| ||
Cell Rep Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.
| ||
