×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 87770-3-RR

    WB analysis using 87770-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87770-3-RR (VAPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 87770-3-RR

    IHC staining of human heart using 87770-3-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 87770-3-RR (VAPB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAPB antibody included
  • IHC staining of human heart using 87770-3-RR

    IHC staining of human heart using 87770-3-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 87770-3-RR (VAPB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAPB antibody included

VAPB Recombinant monoclonal antibody

VAPB Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87770-3-RR

产品说明书

CloneNo. 253359F2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

ALS8, UNQ484/PRO983, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, rat liver tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VAPB Ready-To-Use IHC Kit. VAPB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87770-3-RR targets VAPB in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag5857

Product name: Recombinant human VAPB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-180 aa of BC001712

Sequence: KLGNPTDRNVCFKVKTTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFAPTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 VAMP (vesicle-associated membrane protein)-associated protein B and C
别名 ALS8, UNQ484/PRO983, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C
计算分子量 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC001712
基因名称 VAPB
Gene ID (NCBI) 9217
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95292
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR), and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease, including late-onset spinal muscular atrophy.

实验方案

Product Specific Protocols
IHC protocol for VAPB antibody 87770-3-RRDownload protocol
WB protocol for VAPB antibody 87770-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...