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验证数据展示

  • WB analysis using 67845-1-Ig (same clone as 67845-1-PBS)

    WB analysis using 67845-1-Ig (same clone as 67845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67845-1-Ig (VAPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67845-1-Ig (same clone as 67845-1-PBS)

    WB analysis of HEK-293 using 67845-1-Ig (same clone as 67845-1-PBS)

    WB result of VAPA antibody (67845-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAPA transfected HEK-293 cells. This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

VAPA Monoclonal antibody, PBS Only

VAPA Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67845-1-PBS

产品说明书

CloneNo. 1F10F9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

1F10F9, 33 kDa VAMP associated protein, 33 kDa VAMP-associated protein, hVAP 33, VAMP A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67845-1-PBS targets VAPA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7819

Product name: Recombinant human VAPA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-221 aa of BC002992

Sequence: MASASGAMAKHEQILVLDPPTDLKFKGPFTDVVTTNLKLRNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGSTVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNTSDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASFR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
别名 1F10F9, 33 kDa VAMP associated protein, 33 kDa VAMP-associated protein, hVAP 33, VAMP A
计算分子量 28 kDa
观测分子量 33 kDa
GenBank蛋白编号BC002992
基因名称 VAPA
Gene ID (NCBI) 9218
RRIDAB_2918607
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9P0L0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

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