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验证数据展示

  • WB analysis using 87278-3-RR (same clone as 87278-3-PBS)

    WB analysis using 87278-3-RR (same clone as 87278-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87278-3-RR (AOC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87278-3-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 87278-3-RR (same clone as 87278-3-PBS)

    IHC staining of human kidney using 87278-3-RR (same clone as 87278-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 87278-3-RR (VAP1/AOC3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87278-3-PBS in a different storage buffer formulation.

  • Affinity of 87278-3-RR

    Affinity of 87278-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87278-3-RR against Human VAP1/AOC3 were performed. The affinity constant is below 1 pM.

VAP1/AOC3 Recombinant monoclonal antibody, PBS Only

VAP1/AOC3 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87278-3-PBS

产品说明书

CloneNo. 252158A7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

AOC3, VAP1, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87278-3-PBS targets VAP1/AOC3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg5045

Product name: Recombinant Human AOC3 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 27-763 aa of NM_003734.3

Sequence: GRGGDGGEPSQLPHCPSVSPSAQPWTHPGQSQLFADLSREELTAVMRFLTQRLGPGLVDAAQARPSDNCVFSVELQLPPKAAALAHLDRGSPPPAREALAIVFFGRQPQPNVSELVVGPLPHPSYMRDVTVERHGGPLPYHRRPVLFQEYLDIDQMIFNRELPQASGLLHHCCFYKHRGRNLVTMTTAPRGLQSGDRATWFGLYYNISGAGFFLHHVGLELLVNHKALDPARWTIQKVFYQGRYYDSLAQLEAQFEAGLVNVVLIPDNGTGGSWSLKSPVPPGPAPPLQFYPQGPRFSVQGSRVASSLWTFSFGLGAFSGPRIFDVRFQGERLVYEISLQEALAIYGGNSPAAMTTRYVDGGFGMGKYTTPLTRGVDCPYLATYVDWHFLLESQAPKTIRDAFCVFEQNQGLPLRRHHSDLYSHYFGGLAETVLVVRSMSTLLNYDYVWDTVFHPSGAIEIRFYATGYISSAFLFGATGKYGNQVSEHTLGTVHTHSAHFKVDLDVAGLENWVWAEDMVFVPMAVPWSPEHQLQRLQVTRKLLEMEEQAAFLVGSATPRYLYLASNHSNKWGHPRGYRIQMLSFAGEPLPQNSSMARGFSWERYQLAVTQRKEEEPSSSSVFNQNDPWAPTVDFSDFINNETIAGKDLVAWVTAGFLHIPHAEDIPNTVTVGNGVGFFLRPYNFFDEDPSFYSADSIYFRGDQDAGACEVNPLACLPQAAACAPDLPAFSHGGFSHN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 amine oxidase, copper containing 3 (vascular adhesion protein 1)
别名 AOC3, VAP1, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase
计算分子量85 kDa
观测分子量 85-90 kDa
GenBank蛋白编号NM_003734.3
基因名称 AOC3
Gene ID (NCBI) 8639
RRIDAB_3745442
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16853-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

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