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VAMP5 Polyclonal antibody

VAMP5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11822-1-AP

Print datasheet

Synonyms

Myobrevin, VAMP 5, VAMP5

验证数据展示

  • WB analysis of human lung using 11822-1-AP

    WB analysis of human lung using 11822-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 11822-1-AP (VAMP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11822-1-AP

    IHC staining of human lung cancer using 11822-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11822-1-AP (VAMP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAMP5 antibody included
  • IHC staining of human lung cancer using 11822-1-AP

    IHC staining of human lung cancer using 11822-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11822-1-AP (VAMP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAMP5 antibody included

经过测试的应用

Positive WB detected inhuman lung tissue, mouse kidney tissue, Human peripheral blood leukocyte cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VAMP5 Ready-To-Use IHC Kit. VAMP5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11822-1-AP targets VAMP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen VAMP5 fusion protein Ag2390 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vesicle-associated membrane protein 5 (myobrevin)
Synonyms Myobrevin, VAMP 5, VAMP5
Calculated Molecular Weight 12.8 kDa
Observed Molecular Weight 13-15 kDa
GenBank Accession NumberBC017891
Gene Symbol VAMP5
Gene ID (NCBI) 10791
RRIDAB_2212965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95183
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAMP5, also known as myobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP5 may participate in trafficking events that are associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking.

实验方案

Product Specific Protocols
WB protocol for VAMP5 antibody 11822-1-APDownload protocol
IHC protocol for VAMP5 antibody 11822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Immunol

Immune Gene Signatures and Immunotypes in Immune Microenvironment Are Associated With Glioma Prognose.

Authors - Xiang-Xu Wang
View Article
humanWB

Front Mol Neurosci

Construction and validation of a risk model based on the key SNARE proteins to predict the prognosis and immune microenvironment of gliomas

Authors - Luxin Yin
View Article