• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

VAMP4 Monoclonal antibody

VAMP4 Monoclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat, Mouse and More (1)

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

3A6F2

Cat No : 67219-1-Ig

Print datasheet

Synonyms

VAMP 4, VAMP24, VAMP4

验证数据展示

  • WB analysis using 67219-1-Ig

    WB analysis using 67219-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67219-1-Ig (VAMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67219-1-Ig

    IHC staining of mouse brain using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67219-1-Ig

    IHC staining of mouse brain using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 67219-1-Ig

    IF Staining of HEK-293 using 67219-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67219-1-Ig (VAMP4 antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67219-1-Ig targets VAMP4 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsIF
Tested Reactivity Human, Pig, Rat, Mouse
Cited Reactivitymouse
Immunogen VAMP4 fusion protein Ag27854 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name vesicle-associated membrane protein 4
Synonyms VAMP 4, VAMP24, VAMP4
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC007019
Gene Symbol VAMP4
Gene ID (NCBI) 8674
RRIDAB_2882510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

实验方案

Product Specific Protocols
WB protocol for VAMP4 antibody 67219-1-IgDownload protocol
IHC protocol for VAMP4 antibody 67219-1-IgDownload protocol
IF protocol for VAMP4 antibody 67219-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

eNeuro

ADP ribosylation factor 4 (Arf4) regulates radial migration through N-cadherin trafficking during cerebral cortical development

Authors - Yoshinobu Hara
View Article