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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 67219-1-Ig

    WB analysis using 67219-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67219-1-Ig (VAMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67219-1-Ig

    IF Staining of HEK-293 using 67219-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67219-1-Ig (VAMP4 antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of rat brain using 67219-1-Ig

    IHC staining of rat brain using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAMP4 antibody included
  • IHC staining of rat brain using 67219-1-Ig

    IHC staining of rat brain using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAMP4 antibody included
  • IHC staining of mouse cerebellum using 67219-1-Ig

    IHC staining of mouse cerebellum using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAMP4 antibody included
  • IHC staining of mouse cerebellum using 67219-1-Ig

    IHC staining of mouse cerebellum using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAMP4 antibody included

VAMP4 Monoclonal antibody

VAMP4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67219-1-Ig

产品说明书

CloneNo. 3A6F2
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

3A6F2, VAMP-4, Vesicle-associated membrane protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy VAMP4 Ready-To-Use IHC Kit. VAMP4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67219-1-Ig targets VAMP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat, pig
文献引用反应性mouse
免疫原

CatNo: Ag27854

Product name: Recombinant human VAMP4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-140 aa of BC007019

Sequence: MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 vesicle-associated membrane protein 4
别名 3A6F2, VAMP-4, Vesicle-associated membrane protein 4
计算分子量 16 kDa
观测分子量 18 kDa
GenBank蛋白编号BC007019
基因名称 VAMP4
Gene ID (NCBI) 8674
RRIDAB_2882510
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75379
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

实验方案

Product Specific Protocols
WB protocol for VAMP4 antibody 67219-1-IgDownload protocol
IHC protocol for VAMP4 antibody 67219-1-IgDownload protocol
IF protocol for VAMP4 antibody 67219-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

eNeuro

ADP ribosylation factor 4 (Arf4) regulates radial migration through N-cadherin trafficking during cerebral cortical development

Authors - Yoshinobu Hara
View Article