验证数据展示
经过测试的应用
| Positive WB detected in | Mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| ELISA | See 3 publications below |
产品信息
30001-0-AP targets VAMP2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Goat / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vesicle-associated membrane protein 2 (synaptobrevin 2) |
| 别名 | SYB2, Synaptobrevin 2, VAMP 2, VAMP2 |
| 计算分子量 | 13 kDa |
| GenBank蛋白编号 | NM_014232 |
| 基因名称 | VAMP2 |
| Gene ID (NCBI) | 6844 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P63027 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family of proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2 consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biochem The biological activity of ubiquitinated BoNT/B light chain in vitro and in human SHSY-5Y neuronal cells. | ||
J Cell Biochem TCEP treatment reduces proteolytic activity of BoNT/B in human neuronal SHSY-5Y cells. | ||
Mol Neurobiol L116 Deletion in CSPα Promotes α-Synuclein Aggregation and Neurodegeneration |