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验证数据展示

  • WB analysis of human brain using 13115-1-AP (same clone as 13115-1-PBS)

    WB analysis of human brain using 13115-1-AP (same clone as 13115-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13115-1-AP (VAMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 13115-1-AP (same clone as 13115-1-PBS)

    IF Staining of SH-SY5Y using 13115-1-AP (same clone as 13115-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 13115-1-AP (VAMP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13115-1-AP (same clone as 13115-1-PBS)

    IHC staining of mouse brain using 13115-1-AP (same clone as 13115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13115-1-AP (VAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13115-1-AP (same clone as 13115-1-PBS)

    IHC staining of mouse brain using 13115-1-AP (same clone as 13115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13115-1-AP (VAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

VAMP1 Polyclonal antibody, PBS Only

VAMP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13115-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13115-1-PBS targets VAMP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3787

Product name: Recombinant human VAMP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC023286

Sequence: MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vesicle-associated membrane protein 1 (synaptobrevin 1)
别名
计算分子量 117 aa, 13 kDa
观测分子量 13 kDa
GenBank蛋白编号BC023286
基因名称 VAMP1
Gene ID (NCBI) 6843
RRIDAB_2214772
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23763
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAMP1, also named as synaptobrevin 1, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP1 is a part of the SNARE (soluble NSF-attachment protein receptor) complex. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP1 is involved in synaptic vesicle exocytosis, a fundamental step in neurotransmitter release.

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