验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, MDCK cells, mouse kidney tissue, HEK-293 cells, HeLa cells |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
26201-1-AP targets Unc119b in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, Canine samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, Canine |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag24599 Product name: Recombinant mouse Unc119b protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-251 aa of BC008617 Sequence: MSGSNPKAATAGSQAGPGGLVAGKEEKKKAGGGVLNRLKARRQGPPHTPDDGSGAAVTEQELLALDTIRPEHVLRLNRVTENYLCKPEDNVYSIDFTRFKIRDLETGTVLFEIAKPCISDQDQDAEEESVDVDISVGRFVRYQFTPAFLRLRTVGATVEFTVGDRPVTGFRMIERHYFRERLLKTFDFDFGFCIPSSRNTCEHIYEFPQLSEDVIRLMIENPYETRSDSFYFVDNKLVMHNKADYAYNGGQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | unc-119 homolog B (C. elegans) |
| 别名 | AA407659, AI414892, unc 119 homolog B (C. elegans), Unc119b |
| 计算分子量 | 28 kDa |
| 观测分子量 | 28-31 kDa |
| GenBank蛋白编号 | BC008617 |
| 基因名称 | Unc119b |
| Gene ID (NCBI) | 106840 |
| RRID | AB_2880422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8C4B4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mouse Unc119b is related closely to Unc119, a homolog of C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. Mouse Unc119b shares 90% sequence identity with human UNC119b protein. UNC119b is a myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Myristoyl-binding activity of UNC119b is required to localize NPHP3 to the primary cilium (PMID: 22085962).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Unc119b antibody 26201-1-AP | Download protocol |
| WB protocol for Unc119b antibody 26201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
