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验证数据展示

  • WB analysis using 60495-1-Ig

    WB analysis using 60495-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60495-1-Ig (Ubiquilin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 60495-1-Ig

    IF Staining of A431 using 60495-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ubiquilin 2 antibody (60495-1-Ig, Clone: 1A6D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Ubiquilin 2 Monoclonal antibody

Ubiquilin 2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 60495-1-Ig

产品说明书

CloneNo. 1A6D11

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

UBQLN2, hPLIC2, hPLIC 2, Dsk2, Chap1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, LNCaP cells, HeLa cells, MOLT-4 cells, pig brain tissue, rabbit brain tissue, rat brain tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60495-1-Ig targets Ubiquilin 2 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat, pig, rabbit
免疫原

CatNo: Ag20251

Product name: Recombinant human Ubiquilin 2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-624 aa of BC069237

Sequence: MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquilin 2
别名 UBQLN2, hPLIC2, hPLIC 2, Dsk2, Chap1
计算分子量 624 aa, 66 kDa
观测分子量66 kDa
GenBank蛋白编号BC069237
基因名称 UBQLN2
Gene ID (NCBI) 29978
RRIDAB_3670264
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UHD9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Ubiquilin 2 antibody 60495-1-IgDownload protocol
WB protocol for Ubiquilin 2 antibody 60495-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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