验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
23984-1-AP targets UTX in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag20018 Product name: Recombinant human UTX protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1059-1401 aa of BC093868 Sequence: DHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitously transcribed tetratricopeptide repeat, X chromosome |
| 别名 | Lysine-specific demethylase 6A, Lysine specific demethylase 6A, KDM6A, Histone demethylase UTX, EC:1.14.11.68 |
| 计算分子量 | 1401 aa, 154 kDa |
| 观测分子量 | 154 kDa |
| GenBank蛋白编号 | BC093868 |
| 基因名称 | UTX |
| Gene ID (NCBI) | 7403 |
| RRID | AB_2935460 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15550 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KDM6A, also named UTX, mediates the removal of repressive trimethylation of histone H3, it plays a central role in the regulation of posterior development, by regulating HOX gene expression (PubMed:17851529).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UTX antibody 23984-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
