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验证数据展示

  • WB analysis of HeLa using 18830-1-AP

    WB analysis of HeLa using 18830-1-AP

    HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 18830-1-AP (1A6/DRIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 18830-1-AP

    IF Staining of HeLa using 18830-1-AP

    Immunofluorescent analysis of Hela cells, using UTP20 antibody 18830-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 18830-1-AP

    IP experiment of HeLa using 18830-1-AP

    IP result of anti-1A6/DRIM (IP:18830-1-AP, 4ug; Detection:18830-1-AP 1:800) with HeLa cells lysate 4000ug.

1A6/DRIM Polyclonal antibody

1A6/DRIM Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 18830-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

DRIM, Down-regulated in metastasis protein, NNP73, Protein Key 1A6, Protein Key-1A6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18830-1-AP targets 1A6/DRIM in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UTP20, small subunit (SSU) processome component, homolog (yeast)
别名 DRIM, Down-regulated in metastasis protein, NNP73, Protein Key 1A6, Protein Key-1A6
计算分子量 318 kDa
观测分子量 310 kDa
GenBank蛋白编号NM_014503
基因名称 UTP20
Gene ID (NCBI) 27340
RRIDAB_10597541
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75691
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1A6/DRIM has been identified as UTP20, a small subunit processome component, functioning in 18S rRNA processing. 1A6/DRIM was originally identified from the malignant derivative of an immortalized gastric epithelial cell line [1] and was found to be down-regulated in metastatic breast cancer cells (PMID: 21151873, 17498821).

实验方案

Product Specific Protocols
IF protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
IP protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
WB protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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