验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, HeLa cells, HepG2 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17416-1-AP targets UTP15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11161 Product name: Recombinant human UTP15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC013064 Sequence: MLKGGQLLVSLKNHHKTVTCLCLSSSGQRLLSGSLDRKVKVYSTTSYKVVHSFDYAASILSLALAHEDETIVVGMTNGILSVKHRKSEAKKESLPRRRRPAYRTFIKGKNYMKQRDDILINRPAKKHLELYDRDLKHFRISKALDRVLDPTCTIKTPEITVSIIKELNRRGVLANALAGRDEKEISHVLNFLIRNLSQPRFAPVLINAAEIIIDIYLPVIGQSPVVDKKFLLLQGLVEKEIDYQRELLETLGMMDMLFATMRRKEGTSVLEHTSDGFPENKKIES 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UTP15, U3 small nucleolar ribonucleoprotein, homolog (S. cerevisiae) |
| 别名 | NET21, UTP15 |
| 计算分子量 | 285aa,32 kDa; 518aa,58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC013064 |
| 基因名称 | UTP15 |
| Gene ID (NCBI) | 84135 |
| RRID | AB_2304317 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TED0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UTP15 is a U3 snoRNA-associated protein of the Small Subunit Processome (SSU), essential for 18S rRNA biogenesis. UTP15 has 3 isoforms with MW 56, 58 and 37kDa (refer to UniProt). Catalog#17416-1-AP is a rabbit polyclonal antibody raised against the full-length of human UTP15. It specially recognizes 58 and 37kDa isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UTP15 antibody 17416-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
