验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11474-1-AP targets UTP14A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | UTP14A fusion protein Ag2027 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UTP14, U3 small nucleolar ribonucleoprotein, homolog A (yeast) |
| 别名 | U3 small nucleolar RNA-associated protein 14 homolog A, SDCCAG16, NY CO 16, KIAA0266, Antigen NY-CO-16 |
| 计算分子量 | 771 aa, 88 kDa |
| 观测分子量 | 88 kDa |
| GenBank蛋白编号 | BC014987 |
| 基因名称 | UTP14A |
| Gene ID (NCBI) | 10813 |
| RRID | AB_2272800 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BVJ6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UTP14A antibody 11474-1-AP | Download protocol |
| IF protocol for UTP14A antibody 11474-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Effects of the Bowen-Conradi syndrome mutation in EMG1 on its nuclear import, stability and nucleolar recruitment.
| ||
RNA Biol The DExD box ATPase DDX55 is recruited to domain IV of the 28S ribosomal RNA by its C-terminal region. | ||
RNA NSUN6 is a human RNA methyltransferase that catalyzes formation of m5C72 in specific tRNAs. | ||
J Cancer U three protein 14a (UTP14A) promotes tumour proliferation and metastasis via the PERK/eIF2a/GRP78 signalling pathway in oesophageal squamous cell carcinoma.
| ||
Cell Rep UTP18-mediated p21 mRNA instability drives adenoma-carcinoma progression in colorectal cancer |
