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验证数据展示

  • WB analysis using 27578-1-AP (same clone as 27578-1-PBS)

    WB analysis using 27578-1-AP (same clone as 27578-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27578-1-AP (UST antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27578-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 27578-1-AP (same clone as 27578-1-PBS)

    IHC staining of human colon cancer using 27578-1-AP (same clone as 27578-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27578-1-AP (UST antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27578-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 27578-1-AP (same clone as 27578-1-PBS)

    IF Staining of U-251 using 27578-1-AP (same clone as 27578-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using UST antibody (27578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27578-1-PBS in a different storage buffer formulation.

UST Polyclonal antibody, PBS Only

UST Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27578-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2OST, uronyl 2 sulfotransferase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27578-1-PBS targets UST in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25483

Product name: Recombinant human UST protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-406 aa of BC093668

Sequence: PRFLLDLRQYLGNSTYLDDHGPPPSKVLPFPSQVVYNRVGKCGSRTVVLLLRILSEKHGFNLVTSDIHNKTRLTKNEQMELIKNISTAEQPYLFTRHVHFLNFSRFGGDQPVYINIIRDPVNRFLSNYFFRRFGDWRGEQNHMIRTPSMRQEERYLDINECILENYPECSNPRLFYIIPYFCGQHPRCREPGEWALERAKLNVNENFLLVGILEELEDVLLLLERFLPHYFKGVLSIYKDPEHRKLGNMTVTVKKTVPSPEAVQILYQRMRYEYEFYHYVKEQFHLLKRKFGLKSHVSKPPLRPHFFIPTPLETEEPIDDEEQDDEKWLEDIYKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uronyl-2-sulfotransferase
别名 2OST, uronyl 2 sulfotransferase
计算分子量 406 aa, 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC093668
基因名称 UST
Gene ID (NCBI) 10090
RRIDAB_3669611
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2C2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UST (Uronyl 2-sulfotransferase) is an enzyme that catalyzes the transfer of sulfate groups to the 2-position of uronyl residues, such as iduronyl residues in dermatan sulfate and glucuronyl residues in chondroitin sulfate (PMID: 10187838). UST is a critical regulator of melanoma cell adhesion and motility in vitro and in vivo. Abnormal expression or activity of UST has also been associated with certain diseases, such as breast pericanalicular fibroadenoma and acute retinal necrosis (PMID: 25480151).

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