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- KD/KO Validated
USP50 Polyclonal antibody
USP50 Polyclonal Antibody for WB,IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB,IHC,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human hepatocirrhosis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24817-1-AP targets USP50 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB,IHC,ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | USP50 fusion protein Ag19337 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin specific peptidase 50 |
| Synonyms | USP50 |
| Calculated Molecular Weight | 339 aa, 39 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC146493 |
| Gene Symbol | USP50 |
| Gene ID (NCBI) | 373509 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q70EL3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin-specific protease 50 (USP50) belongs to the peptidase C19 family. USP50 has been shown to regulate cell cycle and DNA repair through interaction with the Wee1 protein in transformed human osteosarcoma and human epithelial cell lines. USP50 has also been shown to play a role in inflammasomal function by targeting the adaptor protein (PMID: 29101126). USP50 has 2 isoforms with the molecular mass of 38 and 39 kDa. Sometimes higher molecular weight around 44 kDa can also be observed, which may be a modified variant of USP50.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP50 antibody 24817-1-AP | Download protocol |
| IHC protocol for USP50 antibody 24817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis
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