USP48 Polyclonal antibody

USP48 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12076-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (2)

Deubiquitinating enzyme 48, RAP1GA1, Ubiquitin thiolesterase 48, USP48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12076-1-AP targets USP48 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 USP48 fusion protein Ag2749 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 48
别名 Deubiquitinating enzyme 48, RAP1GA1, Ubiquitin thiolesterase 48, USP48
计算分子量 1035 aa, 119 kDa
观测分子量 119 kDa
GenBank蛋白编号BC013567
基因名称 USP48
Gene ID (NCBI) 84196
RRIDAB_2213840
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UV5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for USP48 antibody 12076-1-APDownload protocol
IHC protocol for USP48 antibody 12076-1-APDownload protocol
IP protocol for USP48 antibody 12076-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Acta Pharm Sin B

Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis

Authors - Can Cheng
mouseWB

FASEB J

Candesartan upregulates angiotensin-converting enzyme 2 in kidneys of male animals by decreased ubiquitination

Authors - Ping Wang
humanCoIP

Sci Rep

RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31

Authors - Wei Tian
humanIHC

Rheumatology (Oxford)

Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β.

Authors - Christine Galant
mouseIP

Eur J Pain

USP48 alleviates bone cancer pain and regulates MrgC stabilization in spinal cord neurons of male mice

Authors - Jiacheng Yu
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